I-Proteinase K ye-albhamu ye-tritirachium Cas: 39450-01-6 99% Impushana emhlophe
| Inombolo Yekhathalogi | XD90434 |
| Igama Lomkhiqizo | I-Proteinase K ye-albhamu ye-tritirachium |
| I-CAS | 39450-01-6 |
| I-Molecular Formula | I-C29H27N2O12P |
| Isisindo samangqamuzana | 626.50468 |
| Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
| Ikhodi Yentela Evumelanisiwe | 35079090 |
Ukucaciswa Komkhiqizo
| I-Gravity ethize | 40un/mg amaprotheni min |
| Umsebenzi we-Enzyme | 30un/mg min isisindo esomile |
| Ukubukeka | Impushana emhlophe |
| Isivivinyo | ≥99% |
Ucwaningo lwe-genome-wide chromatin immunoprecipitation (ChIP) silethe ukuqonda okubalulekile ukwenziwa kwasendaweni kwe-genomic kwamaprotheni ahlobene ne-chromatin kanye nokuguqulwa kwe-histone.Inani elikhulu ledatha elikhiqizwa yilokhu kuhlaziya, nokho, lidinga izindlela ezivumela ukuqinisekiswa okusheshayo nokuhlaziywa kokuhambisana kwezinto eziphilayo.Ngaphezu kwalokho, kusekhona amaprotheni nokuguqulwa okuhlosiwe okunzima ukukubona kusetshenziswa izindlela ezijwayelekile ze-ChIP.Ukuze sibhekane nalezi zinkinga, senze inqubo esheshayo ye-chromatin immunoprecipitation esiyibiza ngokuthi i-ZipChip.I-ZipChip inciphisa kakhulu isikhathi futhi ikhulisa ukuzwela okuvumela ukuhlolwa okusheshayo kwezindawo eziningi.Lapha sichaza ukuthi i-ZipChIP ivumela kanjani ukutholwa kokuguqulwa kwe-histone (i-H3K4 mono- ne-trimethylation) kanye ne-yeast histone demethylases, i-Jhd2 ne-Rph1, obekunzima ukuyibona ngaphambilini kusetshenziswa izindlela ezijwayelekile.Ngaphezu kwalokho, sibonisa ukuguquguquka kwe-ZipChIP ngokuhlaziya ukunothiswa kwe-histone deacet ylase Sir2 ku-heterochromatin ku-yeast kanye nokunothisa kwe-chromatin remodeler, i-PICKLE, ku-euchromatin nge-Arabidopsis thaliana.
