I-Uridine,2′-deoxy-5-ethynyl- Cas:61135-33-9
| Inombolo Yekhathalogi | XD90589 |
| Igama Lomkhiqizo | I-Uridine, i-2'-deoxy-5-ethynyl- |
| I-CAS | 61135-33-9 |
| I-Molecular Formula | I-C11H12N2O5 |
| Isisindo samangqamuzana | 252.23 |
| Imininingwane Yesitoreji | -20°C |
| Ikhodi Yentela Evumelanisiwe | 29349990 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Okumhlophe kuya ku-Orenji kuya kumpushana Okuhlaza kuya kukristalu |
| Isivivinyo | ≥99% |
Lolu cwaningo lwenziwa ukuze kuphenywe umphumela okhulayo wemifino ye-soy peptide kumaseli ama-stem cells (ASCs) lapho ingekho i-serum kanye nezindlela zabo zokusebenza okungenzeka.Ukwanda kwamaseli we-mesenchymal stem cell (ADSC) wezicubu ze-adipose (ama-ADSC) kanye namaseli e-mesenchymal stem atholakala egazini (CB-MSCs) aphathwe nge-soy peptides kutholakale ukuthi kwenyuke kakhulu ku-3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide kanye ne-Click-iT 5-ethynyl-2'-deoxyuridine flow cytometry assay.Ngaphezu kwalokho, ama-soy peptides aholele ekungeneni kwe-phosphorylation ye-p44/42 MAPK (ERK), okuqondiwe kwezilwane ezincelisayo kwe-rapamycin (mTOR), i-p70 S6 kinase, i-S6 ribosomal protein (S6RP) kanye ne-eukaryotic initiation factor 4E (eIF4E) kuma-ADSC.Ngaphezu kwalokho, ukuhlaziya okulinganiselwe kwama-cytokines kwembula ukuthi ukukhiqizwa kokushintsha ukukhula kwe-beta1 (TGF-β1), i-vascular endothelial growth factor kanye ne-interleukin-6 kukhuphuke kakhulu ekuphenduleni ukwelashwa ngama-soy peptide kokubili ama-ADSC kanye nama-CB-MSC.Ngokufanayo, i-phosphorylation ye-soy peptide-induced phosphorylation ye-ERK/mTOR/S6RP/eIF4E indlela ivinjiwe ekuphenduleni ukwelashwa kwangaphambili nge-PD98059, i-ERK inhibitor ethile.Ngaphezu kwalokho, ukuvinjelwa kwe-TGF-β1 nge-PD98059 pretreatment kanye nokwehla okulandelanayo ekwandeni kwe-ADSC kwembula ukuthi i-TGF-β1 idala i-phosphorylation ye-mTOR/S6RP/eIF4E.Sekukonke, imiphumela yalolu cwaningo ibonisa ukuthi ukukhiqizwa okuncike ku-ERK kwe-TGF-β1 kudlala indima ebalulekile ekwandeni kwe-ADSC okubangelwa isoya peptide ngaphansi kwezimo ezingenaserum.Copyright © 2012 Elsevier Inc. Wonke amalungelo agodliwe.
