Imikhiqizo

I-europium(III) kanye ne-terbium(III) complexes, okungukuthi [Eu(dpq)(DMF)2(NO3)3] (1), [Eu(dppz)2(NO3)3] (2), [Tb(dpq) )(DMF)2Cl3] (3), kanye ne-[Tb(dppz)(DMF)2Cl3] (4), lapho i-dipyrido[3,2-d:2',3'-f]quinoxaline (dpq ku-1 no-3) , i-dipyrido[3,2-a:2',3'-c]phenazine (dppz ku-2 no-4) kanye ne-N,N'-dimethylformamide (DMF) ahlukaniswe, abonakala kudatha yawo ye-physicochemical, izifundo ze-luminescence kanye nokusebenzisana kwazo. nge-DNA, amaprotheni e-serum albumin kanye nomsebenzi we-DNA cleavage owenziwe ngesithombe kuyafundwa.Izakhiwo zekristalu ze-X-ray ze-complexes 1-4 zibonisa izakhiwo ezisekelwe ku-mononuclear Ln(3+) ehlukene.I-Eu(3+) ku-[Eu(dpq)(DMF)2(NO3)3] (1) kanye ne-[Eu(dppz)2(NO3)3] (2) njenge-[Eu(dppz)2(NO3)3) ]·dppz (2a) yamukela isakhiwo se-dodecahedron ene-bicahedron ene-bidentate N,N-donor dpq ligand, i-DMF emibili kanye nama-anion amathathu e-NO3(-) ku-1 kanye nama-bidentate amabili e-N,N-donor dppz ligand kanye ne-NO3 emithathu( -) ama-anion ku-2. Izinkimbinkimbi 3 kanye no-4 zibonisa ukwakheka kwe-octahedron ene-mono-capped eyisikhombisa lapho i-Tb(3+) iqukethe ama-bidentate dpq/dppz ligands, i-DMF emibili kanye nama-anion amathathu e-Cl(-).Izinkimbinkimbi zikhanya kakhulu ngokwemvelo okubonisa ukudluliswa kwamandla okunenjabulo kwesithombe ukusuka ku-dpq/dppz antenna kuya ku-Ln(3+) ukuze kukhiqizwe izimo zenjabulo eziphila isikhathi eside zoshintsho lwesici f → f.I-spectra ye-luminescence exazululwe isikhathi yamakhompleksi 1-4 ibonisa amabhendi akhiphayo amancane amancane abalulwe ku-(5)D0 → (7)F(J) kanye (5)D4 → (7)F(J) ff izinguquko ze-Eu(3 +) kanye ne-Tb(3+) ions ngokulandelanayo.Inombolo yama-molecule wamanzi e-inner-sphere (q) yanqunywa kusukela kuzilinganiso zesikhathi sempilo ye-luminescence ku-H2O kanye ne-D2O eqinisekisa ukusabela kwe-ligand-exchange ngamanzi ayisixazululo.Izinkimbinkimbi zibonisa ukuthambekela okubalulekile kokubophezela ku-CT-DNA enikeza amanani abophayo angaguquki ebangeni elingu-1.0 × 10(4)-6.1 × 10(4) M(-1) ngohlelo 2, 4 (dppz) > 1, 3 (dpq).Idatha ebophezelayo ye-DNA iphakamisa ukuthi i-DNA groove ibophezeleke nengxenye yemvelo ye-intercalation yezakhiwo.Wonke ama-complexes aphinde abonise ukuthambekela okubophayo (K(BSA) ~ 10(5) M(-1)) kuphrotheni ye-serum yezinkomo (BSA).Amandla amabhendi e-spectral e-lumescence e-luminescence angena isikhathi athuthuka kakhulu ngokukhula kwe-DNA endaweni enomanzi ngenxa yokugudluzwa kwamanzi abophekile lapho ehlangana ne-DNA, ngaleyo ndlela kunciphisa ukucisha okungashisi nge-OH oscillator.I-Complex 1-4 ihlanganisa kahle i-supercoiled (SC) ds-DNA kufomu layo eliyindingilizi eli-nicked (NC) lapho lichayeka ku-UV-A ukukhanya okungu-365 nm ngokwakhiwa kwe-singlet oxygen ((1)O2) kanye nama-hydroxyl radicals (HO˙) njenge izinhlobo ze-oxygen esebenzayo ekugxilweni kwe-micromolar ngaphansi kwezimo zomzimba.