i-oomassie eluhlaza okwesibhakabhaka ecwebezelayo R-250 Cas: 6104-59-2 Impushana eluhlaza okwesibhakabhaka ukuya kumnyama omnyama
| Inombolo Yekhathalogi | XD90541 |
| Igama Lomkhiqizo | oomassie brilliant blue R-250 |
| I-CAS | 6104-59-2 |
| I-Molecular Formula | I-C45H44N3NaO7S2 |
| Isisindo samangqamuzana | 825.97 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 32129000 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana eluhlaza okwesibhakabhaka ukuya kokumnyama |
Sifunde ngokususwa kombala kwe-malachite eluhlaza (MG) yi-fungus Cunninghamella elegans.Umsebenzi we-mitochondrial wokunciphisa i-MG wanda ngokunyuka ngesikhathi esisodwa kweprotheni ye-9-kDa, ebizwa ngokuthi i-CeCyt.Ukuba khona kwe-cytochrome c kuphrotheni ye-CeCyt kwanqunywa i-optical absorbance spectroscopy ene-coefficient yokushabalala (E(550-535)) engu-19.7+/-6.3 mM(-1) cm(-1) namandla okunciphisa angu-+261 mV.Lapho i-CeCyt ehlanziwe yengezwa ku-mitochondria, umsebenzi othize we-CeCyt ufinyelele ku-440 +/- 122 micromol min(-1) mg(-1) amaprotheni.Ukuvinjwa kokuncipha kwe-MG nge-stigmatellin, kodwa hhayi nge-antimycin A, kubonise ukuxhumana okungenzeka komsebenzi we-CeCyt endaweni ye-Qo ye-bc1 complex.Imiphumela ye-RT-PCR ibonise ukulawulwa okuqinile kokubonakaliswa kofuzo lwe-cecyt ngezinhlobo ze-oxygen esebenzayo.Siphakamisa ukuthi i-CeCyt isebenze njenge-protein reductant ye-MG ngaphansi kwengcindezi ye-oxidative esigabeni sokukhula esimile noma sesibili salesi sikhunta.2009 Elsevier Inc. Wonke amalungelo agodliwe.
